| Title | Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius. |
| Publication Type | Journal Article |
| Year of Publication | 2016 |
| Authors | Rosenfeld JA, Reeves D, Brugler MR, Narechania A, Simon S, Durrett R, Foox J, Shianna K, Schatz MC, Gandara J, Afshinnekoo E, Lam ET, Hastie AR, Chan S, Cao H, Saghbini M, Kentsis A, Planet PJ, Kholodovych V, Tessler M, Baker R, DeSalle R, Sorkin LN, Kolokotronis S-O, Siddall ME, Amato G, Mason CE |
| Journal | Nat Commun |
| Volume | 7 |
| Pagination | 10164 |
| Date Published | 2016 Feb 02 |
| ISSN | 2041-1723 |
| Keywords | Animals, Bedbugs, Blood, Chromosome Mapping, Eating, Female, Gene Expression Profiling, Gene Expression Regulation, Developmental, Humans, Life Cycle Stages, Male, Models, Molecular, Phylogeny, Sequence Analysis, RNA |
| Abstract | The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations. |
| DOI | 10.1038/ncomms10164 |
| Alternate Journal | Nat Commun |
| PubMed ID | 26836631 |
| PubMed Central ID | PMC4740774 |
| Grant List | F31 GM111053 / GM / NIGMS NIH HHS / United States R01NS076465 / NS / NINDS NIH HHS / United States P30 CA008748 / CA / NCI NIH HHS / United States T32 GM083937 / GM / NIGMS NIH HHS / United States F31GM111053 / GM / NIGMS NIH HHS / United States R01 GM092706 / GM / NIGMS NIH HHS / United States R01 NS076465 / NS / NINDS NIH HHS / United States 1T32GM083937 / GM / NIGMS NIH HHS / United States R01GM092706 / GM / NIGMS NIH HHS / United States |