Title | Translaminar synchronous neuronal activity is required for columnar synaptic strengthening in the mouse neocortex. |
Publication Type | Journal Article |
Year of Publication | 2025 |
Authors | Vargas-Ortiz J, Lin L, Martinez VK, Liu R-J, Babij R, Duan ZRan S, Wacks S, Sun L, Wang A, Khan S, Soto-Vargas JLee, GarcĂa NVDe Marco, Che A |
Journal | Nat Commun |
Volume | 16 |
Issue | 1 |
Pagination | 1296 |
Date Published | 2025 Feb 03 |
ISSN | 2041-1723 |
Keywords | Animals, Animals, Newborn, Female, Male, Mice, Mice, Inbred C57BL, Neocortex, Neurons, Pyramidal Cells, Somatosensory Cortex, Synapses |
Abstract | Synchronous neuronal activity is a hallmark of the developing mouse primary somatosensory cortex. While the patterns of synchronous neuronal activity in cortical layer 2/3 have been well described, the source of the robust layer 2/3 activity is still unknown. Using a novel microprism preparation and in vivo 2-photon imaging in neonatal mice, we show that synchronous neuronal activity is organized in barrel columns across layers. Monosynaptic rabies tracing and slice electrophysiology experiments reveal that layer 2/3 pyramidal neurons receive significant layer 5 inputs during the first postnatal week, and silencing layer 5 synaptic outputs results in a significant reduction in spontaneous activity, abnormal sensory-evoked activity and disrupted layer 4-layer 2/3 connectivity. Our results demonstrate that translaminar layer 5-layer 2/3 connectivity plays an important role in synchronizing the developing barrel column to ensure the strengthening of layer 4-layer 2/3 connections, supporting the formation of the canonical cortical organization in barrel cortex. |
DOI | 10.1038/s41467-024-55783-w |
Alternate Journal | Nat Commun |
PubMed ID | 39900899 |
PubMed Central ID | PMC11791040 |
Grant List | K99 NS114166 / NS / NINDS NIH HHS / United States Young Investigator Award / / Brain and Behavior Research Foundation (Brain & Behavior Research Foundation) / R01 NS133434 / NS / NINDS NIH HHS / United States R01 MH110553 / MH / NIMH NIH HHS / United States R00 NS114166 / NS / NINDS NIH HHS / United States R01 MH125006 / MH / NIMH NIH HHS / United States 1R01NS116137 / / U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) / R01 DA059378 / DA / NIDA NIH HHS / United States T32 GM007739 / GM / NIGMS NIH HHS / United States R01 NS116137 / NS / NINDS NIH HHS / United States T32GM007739 / / U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) / F30 MH117939 / MH / NIMH NIH HHS / United States 1R01NS133434 / / U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) / 5F30MH117939 / / U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) / 1R01DA0593578 / / U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse (NIDA) / 2R01MH110553 / / U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) / 1R01MH125006 / / U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) / |