Translaminar synchronous neuronal activity is required for columnar synaptic strengthening in the mouse neocortex.

TitleTranslaminar synchronous neuronal activity is required for columnar synaptic strengthening in the mouse neocortex.
Publication TypeJournal Article
Year of Publication2025
AuthorsVargas-Ortiz J, Lin L, Martinez VK, Liu R-J, Babij R, Duan ZRan S, Wacks S, Sun L, Wang A, Khan S, Soto-Vargas JLee, GarcĂ­a NVDe Marco, Che A
JournalNat Commun
Volume16
Issue1
Pagination1296
Date Published2025 Feb 03
ISSN2041-1723
KeywordsAnimals, Animals, Newborn, Female, Male, Mice, Mice, Inbred C57BL, Neocortex, Neurons, Pyramidal Cells, Somatosensory Cortex, Synapses
Abstract

Synchronous neuronal activity is a hallmark of the developing mouse primary somatosensory cortex. While the patterns of synchronous neuronal activity in cortical layer 2/3 have been well described, the source of the robust layer 2/3 activity is still unknown. Using a novel microprism preparation and in vivo 2-photon imaging in neonatal mice, we show that synchronous neuronal activity is organized in barrel columns across layers. Monosynaptic rabies tracing and slice electrophysiology experiments reveal that layer 2/3 pyramidal neurons receive significant layer 5 inputs during the first postnatal week, and silencing layer 5 synaptic outputs results in a significant reduction in spontaneous activity, abnormal sensory-evoked activity and disrupted layer 4-layer 2/3 connectivity. Our results demonstrate that translaminar layer 5-layer 2/3 connectivity plays an important role in synchronizing the developing barrel column to ensure the strengthening of layer 4-layer 2/3 connections, supporting the formation of the canonical cortical organization in barrel cortex.

DOI10.1038/s41467-024-55783-w
Alternate JournalNat Commun
PubMed ID39900899
PubMed Central IDPMC11791040
Grant ListK99 NS114166 / NS / NINDS NIH HHS / United States
Young Investigator Award / / Brain and Behavior Research Foundation (Brain & Behavior Research Foundation) /
R01 NS133434 / NS / NINDS NIH HHS / United States
R01 MH110553 / MH / NIMH NIH HHS / United States
R00 NS114166 / NS / NINDS NIH HHS / United States
R01 MH125006 / MH / NIMH NIH HHS / United States
1R01NS116137 / / U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) /
R01 DA059378 / DA / NIDA NIH HHS / United States
T32 GM007739 / GM / NIGMS NIH HHS / United States
R01 NS116137 / NS / NINDS NIH HHS / United States
T32GM007739 / / U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) /
F30 MH117939 / MH / NIMH NIH HHS / United States
1R01NS133434 / / U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) /
5F30MH117939 / / U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) /
1R01DA0593578 / / U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse (NIDA) /
2R01MH110553 / / U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) /
1R01MH125006 / / U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) /